Trichoderma reesei, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2234
Coordonnateurs : Mach-Aigner Astrid R., Martzy Roland
1. Ecological Genomics and Evolution of Trichoderma reesei
Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, and Feng Cai
2. Industrial Relevance of Trichoderma reesei as an Enzyme Producer
Amanda J. Fischer, Suchindra Maiyuran, and Debbie S. Yaver
3. The Potential of Synthetic Biologyfor Trichoderma reesei
Roland Martzy and Astrid R. Mach-Aigner
4. Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei
Monika Schmoll and Susanne Zeilinger
5. Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei
Irene Tomico-Cuenca and Christian Derntl
6. Open the Pores: Electroporation for the Transformation of Trichoderma reesei
Franziska Wanka
7. Sexual Crossing of Trichoderma reesei
Rita B. Linke
8. CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei
Gen Zou and Zhihua Zhou
9. The Copper-Controlled RNA Interference System in Trichoderma reesei
Lei Wang, Weixin Zhang, Xiangfeng Meng, and Weifeng Liu
10. Batch Cultivation of Trichoderma reesei
Birgit Jovanović
11. Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations
Nicolas Hardy, Maxime Moreaud, and Fadhel Ben Chaabane
12. Measurement of Cellulase and Xylanase Activities in Trichoderma reesei
Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, and Xin-Qing Zhao
13. Flow Cytometry for Filamentous Fungi
Matthias G. Steiger
14. Molecular Identification of Trichoderma reesei
Mohammad Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, and Irina S. Druzhinina
15. In Vivo Footprinting Analysis in Trichoderma reesei
Alice Rassinger
16. RNA Characterization in Trichoderma reesei
Petra Till
17. Proteomic Profiling of the Secretome of Trichoderma reesei
SoFong Cam Ngan and Siu Kwan Sze
18. Transcriptomics in Trichoderma reesei
Amanda C.C. Antonieto and Roberto N. Silva
19. The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances
Bernhard Seidl, Christoph Bueschl, and Rainer Schuhmacher
20. In Silico Gene Analysis and Oligonucleotide Design for Constructionof Expression Vectors
Bernhard Seiboth
21. PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a
Wan-Chen Li and Ting-Fang Wang
22. TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations
Hou-Cheng Liu, Wan-Chen Li, and Ting-Fang Wang
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 11-2021
Ouvrage de 364 p.
17.8x25.4 cm
Date de parution : 11-2020
Ouvrage de 364 p.
17.8x25.4 cm