Plant MicroRNAs, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1932
Coordonnateur : de Folter Stefan
1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants
Alma Armenta-Medina and C. Stewart Gillmor
2. The Plant MicroRNA Pathway: The Production and Action Stages
Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens
3. Plant miRNA Conservation and Evolution
Lydia Gramzow and Günter Theißen
4. Bioinformatic Analysis of Small RNA Sequencing Libraries
Ricardo A. Chávez Montes, Fabiola Jaimes Miranda, and Stefan de Folter
5. An Overview of miRNA and miRNA Target Analysis Tools
Anna Lukasik and Piotr Zielenkiewicz
6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences
Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma
7. Prediction of Plant miRNA Targets
Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey
8. Isolation and Detection Methods of Plant miRNAs
Pedro Fernando Vera Hernández, Stefan de Folter, and Flor de Fátima Rosas Cárdenas
9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants
Carlos De la Rosa and José Luis Reyes
10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses
Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska
11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization
Marcelino Martínez Núñez, Stefan de Folter, and Flor de Fátima Rosas Cárdenas
12. In Situ Localization of Small RNAs in Plants
Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans
13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs
Andrea Tovar-Aguilar, Karla A. Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I. González-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and Noé Durán-Figueroa
14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy
Andrea Gómez-Felipe and Stefan de Folter
15. Degradome Sequencing in Plants
Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu
16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System
Damien Formey, José Ángel Martín-Rodríguez, and Georgina Hernández
17. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis
Gigi Wong and Anthony A. Millar
18. Mimicry Technology: A Versatile Tool for Small RNA Suppression
Luis Manuel Villar-Martin and Ignacio Rubio-Somoza
19. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants
Alberto Carbonell
20. Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches
Belen Moro, Arantxa M.L Rojas, and Javier F. Palatnik
21. Analysis of Methylation Status of Plant MicroRNAs
Susu Chen and Guodong Ren
22. Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1
Laura Arribas-Hernández, Maria Louisa Vigh, and Peter Brodersen
23. The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs
Shengben Li
24. Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae
Gloria León-Martínez, Edgar Demesa-Arévalo, and Jean-Philippe Vielle-Calzada
25. Detection of Histone Modifications Associated with miRNAs
Sara Hernández-Castellano and Clelia De-la-Peña
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 01-2019
Ouvrage de 363 p.
17.8x25.4 cm
Mots-clés :
Plant sciences; Genetic regulation; Bioinformatics; Plant miRNAs; miRNA function; gene expression
